Polydioxanone/pluronic F127 담체에 유입된 골막기원세포의 조골활성
Osteogenic activity of cultured human periosteal-derived cells in a three dimensional polydioxanone/pluronic F127 scaffold
이진호, 김종렬, 오세행, 김욱규, 박봉욱, 하영술, 변준호, 김덕룡,
소속 상세정보
이진호 ( Lee Jin-Ho ) - 한남대학교 생명나노과학대학 신소재공학과
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
오세행 ( Oh Se-heang ) - 한남대학교 생명나노과학대학 신소재공학과
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 구강악안면외과학교실
하영술 ( Hah Young-Sool ) - 경상대학교병원 임상의학연구소
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 구강악안면외과학교실
김덕룡 ( Kim Deok-Ryong ) - 경상대학교 의학전문대학원 생화학교실
KMID : 0360120090310060478
Abstract
Three-dimensional porous scaffolds play an important role in tissue engineering strategies. They provide a void volume in which vascularization, new tissue formation, and remodeling can occur. Like any grafted materials, the ideal scaffold for bone tissue engineering should be biocompatible without causing an inflammatory response. It should also possess biodegradability, which provides a suitable three-dimensional environment for the cell function together with the capacity for gradual resorption and replacement by host bone tissue. Various scaffolds have already been developed for bone tissue engineering applications, including naturally derived materials, bioceramics, and synthetic polymers. The advantages of biodegradable synthetic polymers include the ability to tailor specific functions. The purpose of this study was to examine the osteogenic activity of periosteal-derived cells in a polydioxanone/pluronic F127 scaffold. Periosteal-derived cells were successfully differentiated into osteoblasts in the polydioxanone/pluronic F127 scaffold. ALP activity showed its peak level at 2 weeks of culture, followed by decreased activity during the culture period. Similar to biochemical data, the level of ALP mRNA in the periosteal-derived cells was also largely elevated at 2 weeks of culture. The level of osteocalcin mRNA was gradually increased during entire culture period. Calcium content was detactable at 1 week and increased in a time-dependent manner up to the entire duration of culture. Our results suggest that polydioxanone/pluronic F127 could be a suitable scaffold of periosteal-derived cells for bone tissue engineering.
키워드
Periosteal-derived cells;Polydioxanone/pluronic F127 scaffold
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